At the Bench: Epigenetics

BIOL-UA 130

Using biochemical and genetic approaches with the yeast Saccharomyces cerevisiae, students characterize a large multisubunit protein complex that modifies chromatin and is involved in gene regulation. Affinity chromatography is used to produce purified preparations of wild-type and mutant protein complexes. The purified protein complexes are compared using a wide variety of biochemical techniques, including silver-stained SDS-PAGE, western blot, colorimetric enzymatic assay, and protein interaction assays. Yeast expressing the same mutants is used in genetic experiments to evaluate the importance of the protein complex in cell growth and gene regulation in the cell.

Format: Laboratory

Prerequisites: MCB1 (BIOL-UA 21) and OrgChem2 (CHEM-UA 226)

Corequisites: None

Location: New York

Equivalent(s): None

Syllabus

Term(s) offered:

Requirements satisfied:
  • Major: Biology Standard Track
      • Upper-Level Elective
          • Laboratory Skills
          • Advanced Biology
  • Major: GPH/Biology
      • Additional Elective
  • Minor: Genetics
      • Elective